Multiple Marrow Cultures from a Single Aspirate

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Multiple Marrow Cultures from a Single Aspirate.

Osgood and Brownlee (1937) described a simple method of bone marrow culture. They employed 20 ml. serum bottles containing a plasma or serum medium diluted with a physiological salt solution. Since the volume of marrow aspirate is limited to 1-2 ml., and it is best to have 1-2,000 cells per ml. of medium, one experiment is usually limited to five or six cultures. This method permits of as many ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1950

ISSN: 0021-9746

DOI: 10.1136/jcp.3.2.157